RNA club

"RNA club" rennais meeting

A multifaceted small RNA repressed by the Catabolite Control Protein A modulates gene expression upon glucose limitation in Staphylococcus aureus

Isabelle Caldelari

Pathogenic bacteria must rapidly adapt to ever-changing environmental signals resulting in metabolism remodeling. The carbon catabolite repression is a global regulatory system, allowing them to express genes involved in utilization and metabolization of the preferred carbon source. In Staphylococcus aureus, the catabolite control protein A (CcpA) regulates the catabolite repressing genes. Here, we have identified a CcpA-dependent small non-coding RNA, RsaI that is inhibited by high glucose concentrations, and this inhibition is required for optimal growth. In the absence of glucose, RsaI represses translation initiation of mRNAs encoding a permease of glucose uptake and the FN3K enzyme that protects proteins against damages caused by high glucose concentrations. RsaI also binds preferentially to the 3’ untranslated region of icaR mRNA, encoding the transcriptional repressor of exopolysaccharide production to enhance the PIA-PNAG synthesis, and to sRNAs responding to the uptake of glucose-6 phosphate or to nitric oxide. Finally, transcriptomic reveals a decreased synthesis of genes involved in carbone catabolism pathway and an activation of genes involved in energy production, fermentation, and nitric oxide detoxification as soon as the repression of RsaI is alleviated. This multifaceted RNA can be considered as a metabolic signature when glucose is scarce and growth is arrested.

 

Meeting organised by Reynald GILLET, Luc PAILLARD et Brice FELDEN

>>Tuesday, April 18, 2019 - 11 am - IGDR conference room (ground floor of Building 4 / Villejean Campus) Rennes - France

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