H2020-MSCA-IF-2018- 844070- GUTPOLAR action
GUTPOLAR action aims at reinforcing the French and European research programs on rare diseases by providing the scientific and medical communities with new paradigms and therapeutic targets. To this end, the ER proposes to combine two models to characterize the trafficking mechanisms underlying intestinal absorption. To reach WP1 of the project, he will use genetics and super-resolution live imaging approaches to characterize the role of the V0-ATPase and its genetic network in intestinal polarity and brush border maintenance. In WP 2, he will study these mechanisms of polarity and brush border maintenance in mammals. To this end, he will first learn mouse intestinal organoids technology through a secondment and then use this model to confirm the role of the V0-ATPase, AP-1 and their network in the maintenance of the absorptive membrane, using imaging techniques.
1. (WP1) We uncovered a new role of the V0-ATPase sector of the V-ATPase complex in the control of intestinal cells trafficking, polarity and brush border maintenance. Notably, V0-ATPase silencing in C. elegans fully recapitulates a rare genetic disorder of the intestinal cells called Microvillus inclusions disease (MVID) (Figure 1)
- Bidaud-Meynard A, Nicolle O, Heck M, Le Cunff Y and Michaux G
A V0-ATPase-dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane.
2019, Development, 146, dev174508; Highlight in Development, 2019, 146: e1102
2. (WP2) Thanks to a secondment in Henner Farin’s lab (Georg Speyer Haus, Frakfurt, Germany), we successfully set up intestinal organoids culture at the IGDR (Figure 2).