Gaêlle Marteil

Study of the regulation mechanisms of the entry and the exit of M phase in Xenopus laevis oocytes.

Abstract:
The research topics of our team are focused on the regulation of M phase using Xenopus laevis as a model organism. Concerning M phase entry, I showed that EP45 protein for "Estrogen regulated protein 45 kDa" acts as an oocyte maturation enhancer. EP45 expression analysis also showed that this protein is synthesized in liver, secreted into the blood and then incorporated into oocytes. The second objective of my thesis was to identify new interactors of the MPF complex, the major regulator of M phase progression, during M phase or M-phase exit. For this purpose, I have used two different approaches in order to perform a CDK1, the catalytic subunit of MPF, cosedimentation assay, combined with 1D SDS-PAGE and mass spectrometry. This study allowed us to identify new potential partners of MPF, such as peroxiredoxin-2, Hsp70, calcineurin. All these results will increase knowledge about the composition and regulation of MPF complex during the progression of M phase.

Financial support:
Ministère

Committee: November 5th, 2010
Serge Hardy
Patrick Cormier
Daniel Fisher
Evelyn Houliston
Jacek Kubiak (co-directeur de thèse)
Laurent Richgard-Parpaillon (co-directeur de thèse)

 

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