Marc TRAMIER Group

R & D Quantitative Fluorescence Microscopy

Marc TRAMIER

Team leader

> Email: marc.tramier@univ-rennes1.fr

> Phone: + 33 (0)2 23 23 54 87

The team aims at developing techniques and methodologies in fluorescence microscopy to study dynamics of protein-protein interactions and biochemical activities in live sample. Team approaches are mainly driven by methodological and technological development, its transfer and its applicability in biology to answer relevant new questions.

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Major publications

Selected Publications

  • Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM.
    Demeautis C, Sipieter F, Roul J, Chapuis C, Padilla-Parra S, Riquet FB, Tramier M.
    Sci Rep. (2017) 7:41026.

    A FRET biosensor reveals spatiotemporal activation and functions of Aurora kinase A in living cells.
    Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M.
    Nat Commun. (2016) 7:12674.

    Golgi sorting regulates organization and activity of GPI proteins at apical membranes.
    Paladino S, Lebreton S, Tivodar S, Formiggini F, Ossato G, Gratton E, Tramier M, Coppey-Moisan M, Zurzolo C.
    Nat Chem Biol. 2014 May.

    Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
    Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
    Methods Mol Biol. 2014.

    Spatio-temporal quantification of FRET in living cells by fast Time-Domain FLIM: a comparative study of non-fitting methods.
    Leray A, Padilla-Parra S, Roul J, Héliot L, Tramier M.
    PLoS One. 2013 Jul.

    FRET microscopy in the living cell: Different approaches, strengths and weaknesses.
    Padilla-Parra S, Tramier M.
    BioEssays. 2012 May.

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