Prigent Group Research

One of the most fascinating events of cell division is mitosis, when the cell distributes its genetic material between its two daughter cells and divides. Two microtubule architectures are essential for the segregation of chromosomes in mitosis: the bipolar spindle (metaphase) and the central spindle (anaphase). We try to understand how are assembled these spindles with a particular focus on the central spindle. We are also interested in the roles of the primary cilia: a cellular structure also assembled from microtubules but on quiescent cells, this cilia is disassembled when the cell enters a division cycle. Our projects are mainly focused on the study of post-translational modifications of proteins that regulate cell cycle progression, such as phosphorylation and ubiquitination. The team is also heavily involved in cancer research and ciliopathies. We seek to understand how deregulation of cell cycle controls can contribute to cancer occurrence, progression, and resistance to treatment. We are looking for inhibitors of the cell cycle proteins to be used in cancer treatments.

 

  Human mitotic spindle visualised by epifluoresence microscopy. After fixation of mitotic cells, the spindle is stained for tubulin to visualise in green the microtubules that insure the dynamic architecture of the spindle, the DNA at the metaphase plate is stained in blue.  Abnormal human cell observed by epifluoresence microscopy. It shows several nuclei and centrosomes, often observed in cancer cells. The cell is fixed and stained for a- and b-tubulin to visualise microtubules in green and for g-tubulin to visualise the centrosomes in red. DNA appears in blue. Cellule humaine dont les deux centrosomes sont marqués en vert (tubuline gamma) et le cil primaire en rouge (tubulaire acétylée)   l'ADN apparaît en bleu.

 

 

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