Third generation sequencing

Long read sequencing : Oxford Nanopore Technology (ONT)

The Long-read sequencing technology proposed by ONT (Oxford Nanopore Technologies) is based on the detection of a differential current as a single linear molecule passes through a biological nanopore anchored in a synthetic membrane. The nanopore is an ion flow sensor that varies according to the steric hindrance of the compound passing through it, which allows to identify the sequence of a DNA strand over a large length (up to several Mb) whatever its composition in repeated elements, GC-rich or methylated regions.

This type of sequencing technology can be used in many biological applications (not exhaustive):

  • Basic research in genomics:
    • Resolve difficult to sequence regions (i.e. repeats) with ultra long reads.
    • Identify RNA/DNA chemical modifications (methylation, acetylation) by direct sequencing.
  • RNA research
    • Characterize and quantify complete transcripts using long reads (direct exon connectivity).
    • Explore epitranscriptomic modifications with direct RNA sequencing.
  • Clinical research
    • Mapping complex structural variants (SV), including insertions of transposable elements.
    • Phase the variants using long readings.
  • Microbiome
    • Assembling complete genomes.
    • Sequencing at the sampling location (taking into account portability)

 

Minit

 

IGDRion Project: MinION@IGDR

This project aims to summarize information about the process of using MinION sequencing technology at IGDR.

The information is also accessible via the dedicated GitLab server : GitLab.

Localization :

The minION is located on the Villejean Campus, Building 4, IGDR, room 221 (2nd floor).

System :

We have 2 MK1B with the Min-IT solution. An MK1C is on order (summer 2021).

 

Minion