Responsable d’Équipe



Responsable d'équipe

En quelques mots

After my PhD in Cell Biology with B. Hoflack at EMBL (European Molecular Biology Laboratory, Heidelberg, Germany), during which I was interested in membrane trafficking, I joined the lab of F. Schweisguth at the Ecole Normale Supérieure (Paris) to combine cell biology and developmental biology approaches. I have been studying asymmetric cell division and Notch dependent binary cell fate acquisition using Drosophila as a model organism. There, I first show that cell-cell contact provide a positional cue to orient asymmetric cell division. Second, I showed that the E3 ubiquitin ligase Neuralized segregates unequally during asymmetric cell division, to control the endocytic trafficking of the Notch ligand, a process essential for binary activation of Notch. I also showed that a second E3 ubiquitin ligase called Mind Bomb has a complementary function to Neuralized during Drosophila development, leading to the concept that Notch activation always requires the endocytosis of its ligands. Since my arrival at the IGDR, my lab is interested in the molecular mechanisms of epithelial cell division, linking the transmission of epithelial barrier functions to the spatio-temporal activation of the Notch pathway during cytokinesis. Since 2013, we are developing biophysical approaches to study the role of tensile forces at the level of adherens junctions in the control of Notch activation at cytokinesis.

Publications majeures

  • Articles

    - N. Loyer, I. Kolotuev, M. Pinot, R. Le Borgne. ‘Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growth. Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12717-22.

    - Cotton, M., Benhra, N., and Le Borgne, R. (2013) Numb inhibits the recycling of Sanpodo in Drosophila sensory organ precursor. Current Biology 23 (7), pp 581-7.Comment in Current Biology 23 (7) R270-2

    -Founounou, N., Loyer, N. and Le Borgne, R. (2013) Septins regulate the contractility of the actomyosin ring to enable adherens junction remodeling during cytokinesis of epithelial cells. (2013) Developmental cell 24 (3), pp242-255. Cited in Faculty of 1000 Biology & comment in Developmental  Cell 24(4) 336-8

    - Benhra, N., Lallet, L., Cotton, M., Le Bras, S., Dussert, A., and Le Borgne, R. (2011). AP-1 controls the trafficking of Notch and Sanpodo toward E-Cadherin junctions in sensory organ precursors. Current  Biology 21 (1), 87-95. Cited in Faculty of 1000 Biology

    - Langevin,L., Le Borgne,R., (equal contribution), Rosenfeld, F., Gho,M., Schweisguth, F. and Bellaïche, Y. (2005). Lethal giant larvae controls the localisation of the Notch signaling regulators Numb, Neuralized and Sanpodo in Drosophila sensory organ precursor cells. Current Biology  15, 955-62 Cited in Faculty of 1000 Biology

    Le Borgne, R., Remaud, S., Hamel,S., and Schweisguth F (2005). The E3 ubiquitin ligases Mind bomb and Neuralized have distinct and complementary functions in the regulation of Delta and Serrate activity in drosophila. PLoS Biology 3, e96. Cited in Faculty of 1000 Biology

    - Le Borgne, R. and Schweisguth, F. (2003). Unequal segregation of Neuralized biases Notch activation during asymmetric cell division. Developmental  Cell  5(1), 139-148. Cited in Faculty of 1000 Biology

    - Le Borgne R., Bellaïche Y.and Schweisguth F.(2002) Drosophila E-Cadherin regulates the orientation of asymetric cell division in the sensory organ lineage, Current Biology, 12, 95-104

    - Le Borgne,R., and Hoflack,B. (1997). The mannose 6-phosphate receptors regulate the formation of clathrin-coated vesicles in the trans-Golgi network. J. Cell Biol. 137: 335-345



    - Le Bras, S. and Le Borgne R. (2014) Epithelial cell division: multiplying without losing touch, Journal of Cell Science 127 (24):5127-37

    - Le Bras, S., Loyer, N. and Le Borgne, R. (2011) The multiple facets of ubiquitination in the regulation of notch signaling pathway. Traffic 12(2), pp 149-61

    - Le Borgne,R. (2006). Regulation of Notch signaling by endocytosis and endosomal sorting. Curr Opin Cell Biol. 18(2), 213-22

    - Le Borgne, R., Bardin, A. and Schweisguth F. (2005) Roles of receptor and ligand endocytosis in the regulation of Notch signaling. Development 132, 1751-62

    - Le Borgne, R. and Schweisguth, F. (2003). Notch signaling: endocytosis makes Delta signal better. Curr. Biol. 23, R273-R275

    - Le Borgne, R. and Hoflack, B. (1998). Mechanisms of protein sorting and coat assembly: insights from the clathrin-coated vesicle pathway. Curr. Opin. Cell Biol. 10: 499-503

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