FUNCTIONAL ANALYSIS OF THE COMPLEX MAP7 / KINESIN-1
Keywords : Cell division, Intracellular transport, Microtubule, Stem cells, Cancer
A PhD position is available in our team « Cytoskeleton and Cell Proliferation » in the IGDR UMR 6290 CNRS – UR1, RENNES.
Regulation of the organization of the microtubule (MT) network is essential for the transport of organelles and vesicles. Multiple pathologies are associated with disregulation of this MT-dependent traffic (neurodegenerative diseases). Moreover similar defects are also involved in the potential of invasiveness of malignant cells. Finally, spindle assembly defects can lead to error in chromosomes segregation, a common feature of cancer cells. Altogether, studying the spatio-temporal regulation of the MT cytoskeleton is crucial for a better understanding of human diseases.
Our team is studying the function of kinesin-1, a motor protein that plays a key-role in MT organization and the transport of vesicles on the microtubule network. Interestingly, an autosomal form of hereditary spastic paraplegia, which is directly linked to vesicle transport failure and brain wiring, is associated with mutation of kinesin-1.
We have recently demonstrated that kinesin-1 recruitment on microtubule is regulated by ensconsin/MAP7 in Drosophila stem cells and oocytes (Metivier et al. Development, 2019). We are looking for a PhD student to investigate how Kinesin-1 and its activator MAP7 cooperate in different cell types (neurons and neural stem cells, S2 cells, oocytes). The applicant will analyze different MAP7 and Kinesin-1 mutants in the regulation of the MT-based intracellular transport and in the organization of the MT networks (Mitosis and interphase) using in vitro and in vivo approaches, combined with cutting edge 4-D microscopy techniques.
Position is available from october 2020. For all enquiries about the position, please contact Dr Régis GIET (firstname.lastname@example.org).